ABSTRACT
Objective To optimize the enzymolysis condition for Acaudina leucoprocta to research lencoprocta the antioxidant activity of its hyoholysate in vivo.Method Response surface methodology (RSM) was employed to optimize the hydrolysis conditions with protamex.The ICR mice were given ig the hydrolysate of Acaudina lencoprocta obtained under optimal conditions.The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of MDA in blood serum and liver in mice were analyzed.Results and Conclusion The best hydrolysis conditions were:the amount of enzyme was 2.02%;enzymolysis temperature was 51.18 ℃;enzymolysis time was 127.81min,hydroxyl radical scavenging rate was 73.10%.The activities of SOD,GSH-Px in hydrolysate were significantly enhanced and the content of MDA was significantly lower,which showed that this hydrolysate had remarkable antioxidation activity.So the enzymolysis technology for Acaudina leucoprocta was optimal.
ABSTRACT
Objective To analyse the nutrients of Acaudina leucoprocta and explore its hypolipidemic effect. Method The nutrients constituents of Acaudina leucoprocta were analyzed by high-performance liquid chromatography,and was compared with Apstichopus japonicus. The hyperlipidemia model was estabolished by feeding SD male mice with high fat feed. The change of liver index and fat coefficient as well as the effects on serum lipid,antioxidant capacity of serum in rats were observed. Results The fat content of Acaudina leucoprocta was only 0.43%,significantly lower than that of Apstichopus japonicus. The content of amino acids in Acaudina leucoprocta was higher than that of Apstichopus japonicus. Acaudina leucoprocta would decrease the liver index,fat coefficient and TC,TG,LDL-C,LDL-C/HDL-C in serum significantly,and increase HDL-C and HDL-C/TC. MDA and NEFA contents were effectively reduced. GSH-Px activitives were enhanced. Conclusion Acaudina leucoprocta is very rich in protein,and low in fat content. It has hypolipidemic and antioxidative effects,and may reduce the oxidative damage caused by high fat diet. Acaudina leucoprocta may be used as a functional food.